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A simple technique for consistently obtaining large single crystals of hen egg‐white lysozyme in a concentration gradient of NiCl 2
Author(s) -
Magay Elena,
Yoon TaeSung
Publication year - 2011
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889810044936
Subject(s) - lysozyme , crystallization , protein crystallization , nucleation , egg white , muramidase , crystallography , chemistry , chromatography , crystal (programming language) , materials science , biochemistry , organic chemistry , computer science , programming language
A simple protocol is reported for consistently obtaining large single crystals of hen egg‐white lysozyme (HEWL) greater than 2 mm in the longest dimension. Comparative crystallization experiments with different sources of HEWL showed that the simple addition of glass capillaries, `crystal hangers', reduced excessive nucleation and resulted in large single crystals regardless of the commercial source of HEWL.