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Applying broadband terahertz time‐domain spectroscopy to the analysis of crystalline proteins: a dehydration study
Author(s) -
Tych Katarzyna M.,
Burnett Andrew D.,
Wood Christopher D.,
Cunningham John E.,
Pearson Arwen R.,
Davies A. Giles,
Linfield Edmund H.
Publication year - 2011
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889810043372
Subject(s) - terahertz time domain spectroscopy , terahertz radiation , terahertz spectroscopy and technology , spectroscopy , absorption (acoustics) , materials science , absorption spectroscopy , tetragonal crystal system , attenuation coefficient , chemistry , optics , analytical chemistry (journal) , crystallography , optoelectronics , crystal structure , physics , organic chemistry , quantum mechanics , composite material
The application of terahertz time‐domain spectroscopy and imaging to the study of proteins in crystalline form is demonstrated. Terahertz time‐domain spectroscopy is particularly sensitive to the long‐range ordering of molecules, with proven utility for the spectroscopy of crystalline biological small molecules. Here, the terahertz time‐domain absorption response of a macromolecular protein single crystal is investigated for the first time. In particular, the effect of dehydration on the terahertz absorption coefficient of tetragonal hen egg white lysozyme crystals is reported.