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Replacing a reservoir solution with desiccant in vapor diffusion protein crystallization screening
Author(s) -
Lu QinQin,
Yin DaChuan,
Chen RuiQing,
Xie SiXiao,
Liu YongMing,
Zhang XianFang,
Zhu Li,
Liu ZhengTang,
Shang Peng
Publication year - 2010
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889810030803
Subject(s) - crystallization , desiccant , protein crystallization , diffusion , reproducibility , materials science , drop (telecommunication) , chemical engineering , crystal (programming language) , chromatography , chemistry , thermodynamics , computer science , physics , composite material , engineering , telecommunications , programming language
This paper presents a modification to the conventional vapor diffusion (hanging‐ or sitting‐drop) technique for protein crystallization screening. In this modified method, the reservoir solution is replaced with a desiccant to allow for a larger range of protein solution concentrations, thereby providing more opportunities for crystal formation. This method was tested in both reproducibility and screening studies, and the results showed that it significantly improves the efficiency and reduces the cost of protein crystallization screens.