Premium
In‐house characterization of protein powder
Author(s) -
Hartmann Christian Grundahl,
Nielsen Ole Faurskov,
Ståhl Kenny,
Harris Pernille
Publication year - 2010
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889810019576
Subject(s) - powder diffraction , diffractometer , diffraction , protein data bank , crystallography , solvent , characterization (materials science) , materials science , protein crystallization , chemistry , optics , physics , crystal structure , protein structure , nanotechnology , organic chemistry , biochemistry , crystallization
X‐ray powder diffraction patterns of lysozyme and insulin were recorded on a standard in‐house powder diffractometer. The experimental powder diffraction patterns were compared with patterns calculated from Protein Data Bank coordinate data. Good agreement was obtained by including straightforward corrections for background, unit‐cell parameters, disordered bulk solvent and geometric factors. In particular the solvent correction was found crucial for a good agreement. A revised Lorentz factor was derived, which gave a minor, but significant, improvement to the fit in the low‐angle region. An attempt to include calculated H‐atom positions did not improve the overall fit and was abandoned. The method devised was shown to be a quick and convenient tool for distinguishing precipitates and polymorphs of proteins.