Growth of large protein crystals by a large‐scale hanging‐drop method | Zendy

Kakinouchi Keisuke | Zendy; Nakamura Tsutomu | Zendy; Tamada Taro | Zendy; Adachi Hiroaki | Zendy; Sugiyama Shigeru | Zendy; Maruyama Mihoko | Zendy; Takahashi Yoshinori | Zendy; Takano Kazufumi | Zendy; Murakami Satoshi | Zendy; Inoue Tsuyoshi | Zendy; Kuroki Ryota | Zendy; Mori Yusuke | Zendy; Matsumura Hiroyoshi | Zendy

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Growth of large protein crystals by a large‐scale hanging‐drop method

Author(s) -

Kakinouchi Keisuke,

Nakamura Tsutomu,

Tamada Taro,

Adachi Hiroaki,

Sugiyama Shigeru,

Maruyama Mihoko,

Takahashi Yoshinori,

Takano Kazufumi,

Murakami Satoshi,

Inoue Tsuyoshi,

Kuroki Ryota,

Mori Yusuke,

Matsumura Hiroyoshi

Publication year - 2010

Publication title -

journal of applied crystallography

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.429

H-Index - 162

ISSN - 1600-5767

DOI - 10.1107/s0021889810015967

Subject(s) - protein crystallization , drop (telecommunication) , mosaicity , crystal (programming language) , crystallization , materials science , crystallography , capillary action , ice crystals , diffraction , crystal growth , chemistry , optics , x ray crystallography , composite material , physics , telecommunications , organic chemistry , computer science , programming language

A method for growing large protein crystals is described. In this method, a cut pipette tip is used to hang large‐scale droplets (maximum volume 200 µl) consisting of protein and precipitating agents. A crystal grows at the vapor–liquid interface; thereafter the grown crystal can be retrieved by droplet–droplet contact both for repeated macroseeding and for mounting crystals in a capillary. Crystallization experiments with peroxiredoxin of Aeropyrum pernix K1 (thioredoxin peroxidase, ApTPx) and hen egg white lysozyme demonstrated that this large‐scale hanging‐drop method could produce a large‐volume crystal very effectively. A neutron diffraction experiment confirmed that an ApTPx crystal (6.2 mm 3 ) obtained by this method diffracted to beyond 3.5 Å resolution.

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