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Simulation of small‐angle X‐ray scattering from thylakoid membranes
Author(s) -
Kirkensgaard J. J. K.,
Holm J. K.,
Larsen J. K.,
Posselt D.
Publication year - 2009
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889809017701
Subject(s) - small angle x ray scattering , thylakoid , scattering , small angle scattering , sampling (signal processing) , physics , computational physics , materials science , optics , chemical physics , chemistry , chloroplast , biochemistry , detector , gene
Small‐angle X‐ray scattering (SAXS) patterns are calculated from a three‐dimensional model of photosynthetic thylakoid membranes. The intricate structure of the thylakoids is represented by sampling random `electron density points' on geometric surfaces. The simulation setup works as a virtual instrument, allowing direct comparison with experimental data. The simulations qualitatively reproduce experimental data and thus clarify the structural origin of the scattering features. This is used to explain recent SAXS measurements and as a guideline for new experiments and future quantitative modeling. The setup has general applicability for model testing purposes when modeling scattering from membrane systems of complex geometries.