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CHAINSAW : a program for mutating pdb files used as templates in molecular replacement
Author(s) -
Stein Norman
Publication year - 2008
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889808006985
Subject(s) - template , residue (chemistry) , protein data bank (rcsb pdb) , computer science , pruning , molecular replacement , sequence (biology) , protein structure , chemistry , biology , stereochemistry , programming language , biochemistry , botany
CHAINSAW is a model editing program for use in molecular replacement. Given a sequence alignment between template and target, it modifies the template structure on a residue‐by‐residue basis by pruning nonconserved residues while leaving conserved residues unchanged. Three degrees of pruning are possible. An internal alignment algorithm is used to cope with any residues that may be missing from the X‐ray structure. CHAINSAW has been successfully used as a tool to assist in solving a number of protein structures.