Premium
Structural characterization of N ‐lignoceroyl (C24:0) sphingomyelin bilayer membranes: a re‐evaluation
Author(s) -
Takahashi Hiroshi,
Hayakawa Tomohiro,
Kawasaki Yuko,
Ito Kazuki,
Fujisawa Tetsuro,
Kodama Michiko,
Kobayashi Toshihide
Publication year - 2007
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s002188980700859x
Subject(s) - small angle x ray scattering , bilayer , lamellar structure , crystallography , phase (matter) , lipid bilayer , membrane , lamellar phase , sphingomyelin , phase transition , materials science , chemistry , scattering , organic chemistry , optics , thermodynamics , biochemistry , physics
Sphingomyelin (SM) is a membrane lipid and plays important roles in signaling, protein trafficking, cell growth and death. The structure of the bilayer of a hydrated highly asymmetric SM, N ‐lignoceroyl (C24:0) SM, has been investigated with X‐ray diffraction (XRD), simultaneous small‐angle X‐ray scattering (SAXS), wide‐angle XRD and SAXS. At temperatures between two endothermic transitions of hydrated C24:0 SM bilayers, the C24:0 SM formed a ripple phase with the ripple periodicity of ~12–14 nm. At about three months incubation at 277 K, the formation of a stable phase with a short lamellar spacing of 5.62 nm was induced. Based upon the structures revealed by this study and the phase behavior, intermolecular interactions between C24:0 SM molecules in the bilayer membrane are discussed.