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Analysis of small‐angle X‐ray scattering data of protein–detergent complexes by singular value decomposition
Author(s) -
Lipfert Jan,
Columbus Linda,
Chu Vincent B.,
Doniach Sebastian
Publication year - 2007
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889807005791
Subject(s) - scattering , singular value decomposition , momentum transfer , small angle x ray scattering , micelle , signal (programming language) , chemistry , momentum (technical analysis) , decomposition , physics , crystallography , computational physics , analytical chemistry (journal) , optics , chromatography , mathematics , computer science , algorithm , finance , organic chemistry , aqueous solution , economics , programming language
Small‐angle X‐ray scattering can be a valuable tool in the structural characterization of membrane protein–detergent complexes (PDCs). However, a major challenge is to separate the PDC scattering signal from that of the `empty' detergent micelle in a protein–detergent mixture. We briefly review an approach that allows approximate determination of the PDC scattering signal at low momentum transfer and present a novel approach that employs a singular value decomposition (SVD) and fitting of scattering data collected at different protein–detergent stoichiometries. The SVD approach allows the scattering profile for the PDC over the entire measured momentum transfer range to be obtained, it is applicable to strongly scattering detergents and can take into account interparticle interference. The two approaches are contrasted and an application to the membrane protein TM0026 from Thermotoga maritima is presented.