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Glucose isomerase from Streptomyces rubiginosus – potential molecular weight standard for small‐angle X‐ray scattering
Author(s) -
Kozak Maciej
Publication year - 2005
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889805010472
Subject(s) - radius of gyration , small angle x ray scattering , gyration , scattering , synchrotron radiation , glucose 6 phosphate isomerase , chemistry , synchrotron , bovine serum albumin , materials science , crystallography , analytical chemistry (journal) , chromatography , polymer , enzyme , optics , physics , biochemistry , organic chemistry , geometry , mathematics
Stability of solutions of glucose isomerase from Streptomyces rubiginosus on long‐term storage and on exposure to synchrotron radiation has been studied by the small‐angle X‐ray scattering (SAXS) method. The values of the radii of gyration and forward scattering do not change significantly on storage and on exposure to synchrotron radiation. The mean value of the radius of gyration characterizing glucose isomerase is R G = 3.27 ± 0.02 nm. For comparison, a SAXS study of monodispersive and aggregated bovine serum albumin (BSA) has been carried out. The results show that glucose isomerase could be a more stable molecular weight standard for SAXS than BSA.