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Use of plastic capillaries for macromolecular crystallization
Author(s) -
Potter Rachel R.,
Hong Young S.,
Ciszak Ewa M.
Publication year - 2004
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889804005242
Subject(s) - crystallization , biomolecule , protein crystallization , macromolecule , thaumatin , diffusion , chemistry , chemical engineering , materials science , glucose 6 phosphate isomerase , nanotechnology , thermodynamics , organic chemistry , biochemistry , physics , engineering , gene , enzyme
Techniques of crystallization of biomolecules in plastic fluorocarbon‐formulated capillaries (Nalgene 870 PFA tubing) are presented. These crystallization methods employ batch, free‐interface liquid–liquid diffusion alone, or a combination with vapor diffusion. The growth of crystals of test proteins such as thaumatin and glucose isomerase, as well as of proteins studied in the laboratory, such as dihydrolipoamide dehydrogenase, has been demonstrated. Once the solutions were loaded in capillaries, they were stored in the tubes in frozen state at cryogenic temperatures until required for crystallization experiments.