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A quick and gentle method for mounting crystals in capillaries
Author(s) -
Basavappa Ravi,
Petri Edward T.,
Tolbert Blanton S.
Publication year - 2003
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889803014006
Subject(s) - capillary action , apprehension , crystal (programming language) , flash (photography) , materials science , optics , computer science , physics , composite material , programming language , psychology , cognitive psychology
The capillary mounting of protein crystals is still necessary under some circumstances, despite its being supplanted by loop mounting/flash freezing as the favored method for crystal mounting prior to data collection. Traditional capillary mounting methods require a degree of dexterity and finesse that causes apprehension among many crystallographers. Here a simple method for capillary mounting that uses a loop for depositing the crystal in the capillary is described.