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Phasing possibilities using different wavelengths with a xenon derivative
Author(s) -
Panjikar Santosh,
Tucker Paul A.
Publication year - 2002
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889802002509
Subject(s) - xenon , phaser , anomalous scattering , wavelength , scattering , synchrotron , phase (matter) , diffraction , optics , chemistry , materials science , computational physics , crystallography , physics , atomic physics , organic chemistry
Xenon derivatives are generally expected to be isomorphous with the native; however, the K ‐ and L ‐absorption edges are not easily accessible on most synchrotron beamlines, which might limit their usefulness in phase determination. Various phasing procedures for xenon‐derivatized porcine pancreatic elastase have been investigated using data sets measured at three generally accessible wavelengths. The importance of highly redundant data in measuring precise anomalous differences is highlighted and it is shown that, after such measurements, a single isomorphous replacement anomalous scattering (SIRAS) procedure yields a better phase set than those generated by single anomalous scattering (SAS) or multiwavelength anomalous diffraction (MAD) procedures.