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Polycapillary optic–source combinations for protein crystallography
Author(s) -
Hofmann F. A.,
Gibson W. M.,
MacDonald C. A.,
Carter D. A.,
Ho J. X.,
Ruble J. R.
Publication year - 2001
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s002188980100468x
Subject(s) - collimated light , collimator , optics , light source , software , materials science , physics , computer science , laser , programming language
The work in this paper is a systematic study of the application of collimating and slightly focusing polycapillary optics to the X‐ray crystallographic structure determination of egg‐white lysozyme using two different sources: a standard rotating anode source and a low‐power table‐top microfocusing source. For the rotating anode source, a series of measurements comparing duplicate data sets obtained from the same individual crystal are summarized. Intensity and data quality are discussed for measurements with a pinhole collimator, a collimating polycapillary optic and a focusing polycapillary optic. The collected data were analyzed using conventional analysis software; limitations of the use of conventional analysis software for focused beams are discussed. Two data sets were collected using the low‐power source and collimating optics, and three data sets using a lower‐power source and focusing optics with three different limiting apertures.