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A simple technique to control macromolecular crystal nucleation efficiently using a standard vapour‐diffusion setup
Author(s) -
Jovine Luca
Publication year - 2000
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889800005136
Subject(s) - nucleation , crystallization , macromolecule , diffusion , crystal (programming language) , materials science , drop (telecommunication) , diffraction , simple (philosophy) , crystallography , chemical physics , chemistry , thermodynamics , optics , computer science , physics , telecommunications , biochemistry , philosophy , epistemology , programming language
Excessive nucleation often leads to a large number of small macromolecular crystals that are not useful for X‐ray diffraction analysis. Crystals with dimensions suitable for data collection can be reproducibly obtained by releasing, for a discrete amount of time, the vapour pressure in both hanging‐ and sitting‐drop experiments, set up at lower precipitant concentrations than those required for crystallization.