A simple technique to control macromolecular crystal nucleation efficiently using a standard vapour‐diffusion setup | Zendy

Jovine Luca | Zendy

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A simple technique to control macromolecular crystal nucleation efficiently using a standard vapour‐diffusion setup

Author(s) -

Jovine Luca

Publication year - 2000

Publication title -

journal of applied crystallography

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.429

H-Index - 162

ISSN - 1600-5767

DOI - 10.1107/s0021889800005136

Subject(s) - nucleation , crystallization , macromolecule , diffusion , crystal (programming language) , materials science , drop (telecommunication) , diffraction , simple (philosophy) , crystallography , chemical physics , chemistry , thermodynamics , optics , computer science , physics , telecommunications , biochemistry , philosophy , epistemology , programming language

Excessive nucleation often leads to a large number of small macromolecular crystals that are not useful for X‐ray diffraction analysis. Crystals with dimensions suitable for data collection can be reproducibly obtained by releasing, for a discrete amount of time, the vapour pressure in both hanging‐ and sitting‐drop experiments, set up at lower precipitant concentrations than those required for crystallization.

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