Examination of Protein Complexes Gets SiMPull
Author(s) -
Jennifer Mach
Publication year - 2016
Publication title -
the plant cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.324
H-Index - 341
eISSN - 1532-298X
pISSN - 1040-4651
DOI - 10.1105/tpc.16.00590
Subject(s) - bimolecular fluorescence complementation , biology , immunoprecipitation , complementation , yeast , protein–protein interaction , computational biology , biophysics , microbiology and biotechnology , genetics , gene , phenotype
Assessing protein-protein interactions remains a fundamental challenge for plant biologists. Current methods such as coimmunoprecipitation (co-IP), yeast two-hybrid, bimolecular fluorescence complementation (BiFC), and others can produce artifacts and also yield only a bulk “ensemble” readout
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