Secretory Pathway Research: The More Experimental Systems the Better | Zendy

Jürgen Denecke | Zendy; Fernando Aniento | Zendy; Lorenzo Frigerio | Zendy; Chris Hawes | Zendy; Inhwan Hwang | Zendy; Jaideep Mathur | Zendy; JeanMarc Neuhaus | Zendy; David G. Robinson | Zendy

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Secretory Pathway Research: The More Experimental Systems the Better

Author(s) -

Jürgen Denecke,

Fernando Aniento,

Lorenzo Frigerio,

Chris Hawes,

Inhwan Hwang,

Jaideep Mathur,

JeanMarc Neuhaus,

David G. Robinson

Publication year - 2012

Publication title -

the plant cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.324

H-Index - 341

eISSN - 1532-298X

pISSN - 1040-4651

DOI - 10.1105/tpc.112.096362

Subject(s) - biology , ectopic expression , computational biology , gene expression , function (biology) , mutant , gene , arabidopsis , microbiology and biotechnology , plant cell , subcellular localization , regulation of gene expression , protoplast , genetics

Transient gene expression, in plant protoplasts or specific plant tissues, is a key technique in plant molecular cell biology, aimed at exploring gene products and their modifications to examine functional subdomains, their interactions with other biomolecules, and their subcellular localization. Here, we highlight some of the major advantages and potential pitfalls of the most commonly used transient gene expression models and illustrate how ectopic expression and the use of dominant mutants can provide insights into protein function.

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