Cautionary Notes on the Use of C-Terminal BAK1 Fusion Proteins for Functional Studies | Zendy

Vardis Ntoukakis | Zendy; Benjamin Schwessinger | Zendy; Cécile Segonzac | Zendy; Cyril Zipfel | Zendy

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Cautionary Notes on the Use of C-Terminal BAK1 Fusion Proteins for Functional Studies

Author(s) -

Vardis Ntoukakis,

Benjamin Schwessinger,

Cécile Segonzac,

Cyril Zipfel

Publication year - 2011

Publication title -

the plant cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.324

H-Index - 341

eISSN - 1532-298X

pISSN - 1040-4651

DOI - 10.1105/tpc.111.090779

Subject(s) - biology , complementation , flagellin , genetics , fusion protein , phenotype , mutant , microbiology and biotechnology , computational biology , receptor , gene , recombinant dna

Detailed phenotypic characterization reveals that several BAK1 fusion proteins with C-terminal tags strongly impair complementation of bak1 null mutants with respect to responsiveness to the bacterial pathogen-associated molecular patterns flagellin and EF-Tu. This raises concerns about the widespread use of such protein variants of this important regulatory Leu-rich repeat receptor-like kinase (RLK) for functional analyses of RLK-based signaling.

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