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Guidelines for Quantitative RT-PCR
Author(s) -
Cathie Martin
Publication year - 2009
Publication title -
the plant cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.324
H-Index - 341
eISSN - 1532-298X
pISSN - 1040-4651
DOI - 10.1105/tpc.109.210480
Subject(s) - notice , biology , frustration , computational biology , mathematics education , psychology , neuroscience , law , political science
Something I notice often in supervising new graduate students is their frustration with the irreproducibility of their biological data. For example, I am asked frequently “how many biological replicates do I need for qRT-PCR”? The answer, of course, lies first with the students themselves. They

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