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Real-Time Quantitative RT-PCR: Design, Calculations, and Statistics
Author(s) -
Ivo Rieu,
Stephen J. Powers
Publication year - 2009
Publication title -
the plant cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.324
H-Index - 341
eISSN - 1532-298X
pISSN - 1040-4651
DOI - 10.1105/tpc.109.066001
Subject(s) - biology , real time polymerase chain reaction , statistics , computational biology , protocol (science) , genetics , gene , mathematics , medicine , alternative medicine , pathology
Two recent letters to the editor of The Plant Cell ([Gutierrez et al., 2008][1]; [Udvardi et al., 2008][2]) highlighted the importance of following correct experimental protocol in quantitative RT-PCR (qRT-PCR). In these letters, the authors outlined measures to allow precise estimation of gene

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