Open AccessTowards a Systematic Validation of References in Real-Time RT-PCR
Author(s) -
Laurent Gutierrez,
Mélanie Mauriat,
Jérôme Pelloux,
Catherine Bellini,
Olivier Van Wuytswinkel
Publication year - 2008
Publication title -
the plant cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.324
H-Index - 341
eISSN - 1532-298X
pISSN - 1040-4651
DOI - 10.1105/tpc.108.059774
Subject(s) - biology , computational biology , real time polymerase chain reaction , genetics , gene
Real-time RT-PCR (also known as quantitative RT-PCR [qRT-PCR]) is a powerful tool for quantifying gene expression, combining both high sensitivity and specificity with efficient signal detection. It has relatively recently begun to be used to monitor gene expression in plants (reviewed in [Gachon et
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