Structural and Functional Analysis of SGT1 Reveals That Its Interaction with HSP90 Is Required for the Accumulation of Rx, an R Protein Involved in Plant Immunity | Zendy

Marta Botër | Zendy; Béatrice Amigues | Zendy; Jack Peart | Zendy; Christian Breuer | Zendy; Yasuhiro Kadota | Zendy; Catarina Casais | Zendy; Geoffrey R. Moore | Zendy; Colin Kleanthous | Zendy; Francoise Ochsenbein | Zendy; Ken Shirasu | Zendy; Raphaël Guérois | Zendy

Open Access

Structural and Functional Analysis of SGT1 Reveals That Its Interaction with HSP90 Is Required for the Accumulation of Rx, an R Protein Involved in Plant Immunity

Author(s) -

Marta Botër,

Béatrice Amigues,

Jack Peart,

Christian Breuer,

Yasuhiro Kadota,

Catarina Casais,

Geoffrey R. Moore,

Colin Kleanthous,

Francoise Ochsenbein,

Ken Shirasu,

Raphaël Guérois

Publication year - 2007

Publication title -

the plant cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.324

H-Index - 341

eISSN - 1532-298X

pISSN - 1040-4651

DOI - 10.1105/tpc.107.050427

Subject(s) - biology , skp1 , hsp90 , arabidopsis , heat shock protein , microbiology and biotechnology , chaperone (clinical) , protein domain , suppressor , protein subunit , genetics , mutant , ubiquitin , gene , ubiquitin ligase , medicine , pathology

SGT1 (for suppressor of G2 allele of skp1) and RAR1 (for required for Mla12 resistance) are highly conserved eukaryotic proteins that interact with the molecular chaperone HSP90 (for heat shock protein90). In plants, SGT1, RAR1, and HSP90 are essential for disease resistance triggered by a number of resistance (R) proteins. Here, we present structural and functional characterization of plant SGT1 proteins. Random mutagenesis of Arabidopsis thaliana SGT1b revealed that its CS (for CHORD-SGT1) and SGS (for SGT1 specific) domains are essential for disease resistance. NMR-based interaction surface mapping and mutational analyses of the CS domain showed that the CHORD II domain of RAR1 and the N-terminal domain of HSP90 interact with opposite sides of the CS domain. Functional analysis of the CS mutations indicated that the interaction between SGT1 and HSP90 is required for the accumulation of Rx, a potato (Solanum tuberosum) R protein. Biochemical reconstitution experiments suggest that RAR1 may function to enhance the SGT1-HSP90 interaction by promoting ternary complex formation.

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