The E3 Ubiquitin Ligase Activity of Arabidopsis PLANT U-BOX17 and Its Functional Tobacco Homolog ACRE276 Are Required for Cell Death and Defense | Zendy

Chengwei Yang | Zendy; Rocío González-Lamothe | Zendy; Richard Ewan | Zendy; Owen Rowland | Zendy; Hirofumi Yoshioka | Zendy; Matthew Shenton | Zendy; Heng Ye | Zendy; Elizabeth O’Donnell | Zendy; Jonathan D. G. Jones | Zendy; Ari Sadanandom | Zendy

Open Access

The E3 Ubiquitin Ligase Activity of Arabidopsis PLANT U-BOX17 and Its Functional Tobacco Homolog ACRE276 Are Required for Cell Death and Defense

Author(s) -

Chengwei Yang,

Rocío González-Lamothe,

Richard Ewan,

Owen Rowland,

Hirofumi Yoshioka,

Matthew Shenton,

Heng Ye,

Elizabeth O’Donnell,

Jonathan D. G. Jones,

Ari Sadanandom

Publication year - 2006

Publication title -

the plant cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.324

H-Index - 341

eISSN - 1532-298X

pISSN - 1040-4651

DOI - 10.1105/tpc.105.039198

Subject(s) - biology , ubiquitin ligase , arabidopsis , pseudomonas syringae , rna interference , hypersensitive response , nicotiana tabacum , arabidopsis thaliana , gene silencing , elicitor , f box protein , ubiquitin , microbiology and biotechnology , genetics , plant disease resistance , mutant , gene , rna

Previous analysis of transcriptional changes after elicitation of Cf-9 transgenic tobacco (Nicotiana tabacum) by Avr9 peptide revealed a rapidly upregulated gene, ACRE276. We show that ACRE276 is transiently induced in wounded leaves within 15 min, but upon Avr9 elicitor treatment, this upregulation is enhanced and maintained until cell death onset in Cf-9 tobacco. ACRE276 RNA interference (RNAi) silencing in tobacco results in loss of hypersensitive response (HR) specified by Cf resistance genes. ACRE276 RNAi plants are also compromised for HR mediated by the tobacco mosaic virus defense elicitor p50. Silencing tomato (Lycopersicon esculentum) ACRE276 leads to breakdown of Cf-9-specified resistance against Cladosporium fulvum leaf mold. We confirmed that tobacco ACRE276 is an E3 ubiquitin ligase requiring an intact U-box domain. Bioinformatic analyses revealed Arabidopsis thaliana PLANT U-BOX17 (PUB17) and Brassica napus ARC1 as the closest homologs of tobacco ACRE276. Transiently expressing PUB17 in Cf-9 tobacco silenced for ACRE276 restores HR, while mutant PUB17 lacking E3 ligase activity fails to do so, demonstrating that PUB17 ligase activity is crucial for defense signaling. Arabidopsis PUB17 knockout plants are compromised in RPM1- and RPS4-mediated resistance against Pseudomonas syringae pv tomato containing avirulence genes AvrB and AvrRPS4, respectively. We identify a conserved class of U-box ARMADILLO repeat E3 ligases that are positive regulators of cell death and defense across the Solanaceae and Brassicaceae.

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