Uniparental Chromosome Elimination at Mitosis and Interphase in Wheat and Pearl Millet Crosses Involves Micronucleus Formation, Progressive Heterochromatinization, and DNA Fragmentation | Zendy

Dorota Gernand | Zendy; Twan Rutten | Zendy; Alok Varshney | Zendy; Myroslava Rubtsova | Zendy; Slaven Prodanović | Zendy; C. Bayan Bruss | Zendy; Jochen Kumlehn | Zendy; Fritz Matzk | Zendy; Andreas Houben | Zendy

Open Access

Uniparental Chromosome Elimination at Mitosis and Interphase in Wheat and Pearl Millet Crosses Involves Micronucleus Formation, Progressive Heterochromatinization, and DNA Fragmentation

Author(s) -

Dorota Gernand,

Twan Rutten,

Alok Varshney,

Myroslava Rubtsova,

Slaven Prodanović,

C. Bayan Bruss,

Jochen Kumlehn,

Fritz Matzk,

Andreas Houben

Publication year - 2005

Publication title -

the plant cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.324

H-Index - 341

eISSN - 1532-298X

pISSN - 1040-4651

DOI - 10.1105/tpc.105.034249

Subject(s) - biology , pennisetum , interphase , chromatin , micronucleus test , chromosome , genetics , mitosis , botany , dna , gene , chemistry , organic chemistry , toxicity

Complete uniparental chromosome elimination occurs in several interspecific hybrids of plants. We studied the mechanisms underlying selective elimination of the paternal chromosomes during the development of wheat (Triticum aestivum) x pearl millet (Pennisetum glaucum) hybrid embryos. All pearl millet chromosomes were eliminated in a random sequence between 6 and 23 d after pollination. Parental genomes were spatially separated within the hybrid nucleus, and pearl millet chromatin destined for elimination occupied peripheral interphase positions. Structural reorganization of the paternal chromosomes occurred, and mitotic behavior differed between the parental chromosomes. We provide evidence for a novel chromosome elimination pathway that involves the formation of nuclear extrusions during interphase in addition to postmitotically formed micronuclei. The chromatin structure of nuclei and micronuclei is different, and heterochromatinization and DNA fragmentation of micronucleated pearl millet chromatin is the final step during haploidization.

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