The Presence of a Chromatin Boundary Appears to Shield a Transgene in Tobacco from RNA Silencing | Zendy

Ľudmila Mlynárová | Zendy; Andrea Hricová | Zendy; Annelies E. H. M. Loonen | Zendy; JanPeter Nap | Zendy

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The Presence of a Chromatin Boundary Appears to Shield a Transgene in Tobacco from RNA Silencing

Author(s) -

Ľudmila Mlynárová,

Andrea Hricová,

Annelies E. H. M. Loonen,

JanPeter Nap

Publication year - 2003

Publication title -

the plant cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.324

H-Index - 341

eISSN - 1532-298X

pISSN - 1040-4651

DOI - 10.1105/tpc.012070

Subject(s) - transgene , biology , gus reporter system , gene , reporter gene , nicotiana tabacum , gene silencing , rna , genetics , genetically modified crops , microbiology and biotechnology , chromatin , gene expression

We present isogenic transgenic tobacco lines that carry at a given chromosomal position a beta-glucuronidase (GUS) reporter gene either with or without the presence of the matrix-associated region known as the chicken lysozyme A element. Plants were generated with the Cre-lox site-specific recombination system using heterospecific lox sites. Analysis of GUS gene expression in plant populations demonstrates that the presence of the A element can shield against RNA silencing of the GUS gene. Protection was observed in two of three independent tobacco transformants. Plants carrying an A element 5' of the GUS gene always had stable GUS activity, but upon removal of this A element, the GUS gene became silenced over time in two lines, notably when homozygous.

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