A Diterpene as an Endogenous Signal for the Activation of Defense Responses to Infection with Tobacco mosaic virus and Wounding in Tobacco | Zendy

Shigemi Seo | Zendy; Hideharu Seto | Zendy; Hiroyuki Koshino | Zendy; Shigeo Yoshida | Zendy; Yuko Ohashi | Zendy

Open Access

A Diterpene as an Endogenous Signal for the Activation of Defense Responses to Infection with Tobacco mosaic virus and Wounding in Tobacco

Author(s) -

Shigemi Seo,

Hideharu Seto,

Hiroyuki Koshino,

Shigeo Yoshida,

Yuko Ohashi

Publication year - 2003

Publication title -

the plant cell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.324

H-Index - 341

eISSN - 1532-298X

pISSN - 1040-4651

DOI - 10.1105/tpc.010231

Subject(s) - biology , tobacco mosaic virus , repressor , gene silencing , rna , gene , microbiology and biotechnology , consensus sequence , genetics , peptide sequence , gene expression , virus

The potato pathogenesis-related gene PR-10a is transcriptionally activated in response to pathogen infection or elicitor treatment. Characterization of the cis-acting elements of the PR-10a promoter revealed the presence of a silencing element between residues −52 and −27 that contributes to transcriptional regulation. In this study, we have isolated a silencing element binding factor (SEBF) from potato tuber nuclei that binds to the coding strand of the silencing element in a sequence-specific manner. The consensus binding site of SEBF, PyTGTCNC, is present in a number of PR genes and shows striking similarity to the auxin response element. Mutational analysis of the PR-10a promoter revealed an inverse correlation between the in vitro binding of SEBF and the expression of PR-10a. SEBF was purified to homogeneity from potato tubers, and sequencing of the N terminus of the protein led to the isolation of a cDNA clone. Sequence analysis revealed that SEBF is homologous with chloroplast RNA binding proteins that possess consensus sequence–type RNA binding domains characteristic of heterogenous nuclear ribonucleoproteins (hnRNPs). Overexpression of SEBF in protoplasts repressed the activity of a PR-10a reporter construct in a silencing element–dependent manner, confirming the role of SEBF as a transcriptional repressor

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research