A Tight-Seal Whole Cell Study of the Voltage-Dependent Gating Mechanism of K+-Channels of Protoplasmic Droplets of Chara corallina | Zendy

Fabrice Homblé | Zendy

Open Access

A Tight-Seal Whole Cell Study of the Voltage-Dependent Gating Mechanism of K⁺-Channels of Protoplasmic Droplets of Chara corallina

Author(s) -

Fabrice Homblé

Publication year - 1987

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.84.2.433

Subject(s) - gating , chara , biophysics , conductance , protoplasm , membrane potential , time constant , tetraethylammonium , chemistry , potassium , analytical chemistry (journal) , materials science , physics , botany , chromatography , biology , electrical engineering , cytoplasm , biochemistry , condensed matter physics , engineering , organic chemistry

The biophysical properties of voltage-dependent K(+)-channels of protoplasmic droplets of Chara corallina Klein ex Willd., em, R.D.W. were investigated using the tight-seal whole cell method. Two potassium currents were observed in voltage-clamp mode and they can be used to explain the transient membrane potential time course observed in current-clamp mode. The K(+)-channels are identified by the effect of tetraethylammonium chloride which blocks both currents. A two-state, constant dipole moment model is used to fit the voltage-conductance curve. From this model the minimum equivalent gating charge involved in the gating mechanism of K(+)-channels of Chara can be estimated.

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