Open AccessA Novel Class of Herbicides (Specific Inhibitors of Imidazoleglycerol Phosphate Dehydratase)
Author(s) -
Ichiro Mori,
R. Fonne-Pfister,
Shigeki Matsunaga,
Sachiyo Tada,
Yoko Kimura,
Genji Iwasaki,
Junichi Mano,
Manabu Hatano,
Toshihito Nakano,
Shinichi Koizumi,
Alfred Scheidegger,
Kenji Hayakawa,
Daisaku Ohta
Publication year - 1995
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.107.3.719
Subject(s) - dehydratase , enzyme , biosynthesis , chemistry , phosphate , histidine , cytotoxicity , biochemistry , stereochemistry , cytotoxic t cell , enzyme inhibition , in vitro
A new mode of herbicidal action was established by finding specific inhibitors of imidazoleglycerol phosphate dehydratase, an enzyme of histidine (His) biosynthesis. Three triazole phosphonates inhibited the reaction of the enzyme with Ki values of 40 [plus or minus] 6.5, 10 [plus or minus] 1.6, and 8.5 [plus or minus] 1.4 nM, respectively, and were highly cytotoxic to cultured plant cells. This effect was completely reversed by the addition of His, proving that the cytotoxicity was primarily caused by the inhibition of His biosynthesis. These inhibitors showed wide-spectrum, postemergent herbicidal activity at application rates ranging from 0.05 to 2 kg/ha.