Detection of a Cytosolic Glutamine Synthetase in Leaves of Nicotiana tabacum L. by Immunocytochemical Methods | Zendy

Helena Carvalho | Zendy; Susana Pereira | Zendy; Cláudio E. Sunkel | Zendy; R. Salema | Zendy

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Detection of a Cytosolic Glutamine Synthetase in Leaves of Nicotiana tabacum L. by Immunocytochemical Methods

Author(s) -

Helena Carvalho,

Susana Pereira,

Cláudio E. Sunkel,

R. Salema

Publication year - 1992

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.100.3.1591

Subject(s) - nicotiana tabacum , glutamine synthetase , chloroplast , cytosol , biology , solanaceae , biochemistry , glutamine , nicotiana , enzyme , microbiology and biotechnology , botany , gene , amino acid

Two glutamine synthetase (GS) polypeptides (44 and 39 kD) were immunodetected on western blots of leaf extracts from tobacco (Nicotiana tabacum L.), a plant that has been reported to contain only chloroplast GS in the leaves. By immunocytochemical methods, we confirmed the localization of GS in the cytosol of cells in the vascular tissue and in the chloroplasts of mesophyll cells.

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