In Situ Nuclear Magnetic Resonance of 15N Pulse Labels Monitors Different Routes for Nitrogen Assimilation | Zendy

R. Callies | Zendy; Rolf Altenburger | Zendy; S. Abarzua | Zendy; Adalbert Mayer | Zendy; L. Horst Grimme | Zendy; Dieter Leibfritz | Zendy

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In Situ Nuclear Magnetic Resonance of ¹⁵N Pulse Labels Monitors Different Routes for Nitrogen Assimilation

Author(s) -

R. Callies,

Rolf Altenburger,

S. Abarzua,

Adalbert Mayer,

L. Horst Grimme,

Dieter Leibfritz

Publication year - 1992

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.100.3.1584

Subject(s) - in situ , glutamate synthase , nitrogen , nitrogen assimilation , assimilation (phonology) , nuclear magnetic resonance , chemistry , glutamate dehydrogenase , chlorella , biochemistry , biology , glutamate receptor , botany , enzyme , algae , physics , receptor , linguistics , philosophy , organic chemistry

Nuclear magnetic resonance offers the possibility of noninvasive in situ observation of (15)N pulse labeling in the presence of light. In vivo, exclusively the delta-nitrogen of Gln is labeled in the cyanobacterium Microcystis firma when glutamate synthase is inhibited by azaserine. In contrast, the green alga Chlorella fusca is additionally capable of incorporating nitrogen into Glu, thus providing evidence for an anabolic function of glutamate dehydrogenase in this organism.

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