Open AccessAnimal Maintenance Systems: Xenopus laevis
Author(s) -
Nikko-Ideen Shaidani,
Sean McNamara,
Marcin Wlizla,
Marko E. Horb
Publication year - 2020
Publication title -
cold spring harbor protocols
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.674
H-Index - 51
eISSN - 1940-3402
pISSN - 1559-6095
DOI - 10.1101/pdb.prot106138
Subject(s) - xenopus , modular design , water quality , aquaculture , biochemical engineering , environmental science , computer science , biology , chemistry , fish <actinopterygii> , fishery , ecology , engineering , biochemistry , gene , operating system
Modular recirculating animal aquaculture systems incorporate UV sterilization and biological, mechanical, and activated carbon filtration, creating a nearly self-contained stable housing environment for Xenopus laevis Nonetheless, minimal water exchange is necessary to mitigate accumulation of metabolic waste, and regular weekly, monthly, and yearly maintenance is needed to ensure accurate and efficient operation. This protocol describes the methods for establishing a new recirculating system and the necessary maintenance, as well as water quality parameters, required for keeping Xenopus laevis .