Calculating the Degradation Rate of Individual Proteins Using Xenopus Extract Systems | Zendy

Gary S. McDowell | Zendy; Anna Philpott | Zendy

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Calculating the Degradation Rate of Individual Proteins Using Xenopus Extract Systems

Author(s) -

Gary S. McDowell,

Anna Philpott

Publication year - 2018

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot103481

Subject(s) - xenopus , degradation (telecommunications) , in vitro , microbiology and biotechnology , protein degradation , embryo , chemistry , computational biology , biology , biochemistry , computer science , gene , telecommunications

The Xenopus extract system has been used extensively as a simple, quick, and robust method for assessing the stability of proteins against proteasomal degradation. In this protocol, methods are provided for assessing the half-life of in vitro translated radiolabeled proteins using Xenopus egg or embryo extracts.

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