The role of insulators and transcription in 3D chromatin organization of flies | Zendy

Keerthi T. Chathoth | Zendy; Liudmila A Mikheeva | Zendy; Gilles Crevel | Zendy; Jareth C. Wolfe | Zendy; Ioni Hunter | Zendy; Saskia Beckett-Doyle | Zendy; Sue Cotterill | Zendy; Hongsheng Dai | Zendy; Andrew Harrison | Zendy; Nicolae Radu Zabet | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

The role of insulators and transcription in 3D chromatin organization of flies

Author(s) -

Keerthi T. Chathoth,

Liudmila A Mikheeva,

Gilles Crevel,

Jareth C. Wolfe,

Ioni Hunter,

Saskia Beckett-Doyle,

Sue Cotterill,

Hongsheng Dai,

Andrew Harrison,

Nicolae Radu Zabet

Publication year - 2022

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.275809.121

Subject(s) - biology , chromatin , euchromatin , transcriptome , gene , transcription factor , genetics , regulation of gene expression , histone , transcription (linguistics) , microbiology and biotechnology , gene expression , computational biology , heterochromatin , linguistics , philosophy

The DNA in many organisms, including humans, is shown to be organized in topologically associating domains (TADs). In Drosophila , several architectural proteins are enriched at TAD borders, but it is still unclear whether these proteins play a functional role in the formation and maintenance of TADs. Here, we show that depletion of BEAF-32, Cp190, Chro, and Dref leads to changes in TAD organization and chromatin loops. Their depletion predominantly affects TAD borders located in regions moderately enriched in repressive modifications and depleted in active ones, whereas TAD borders located in euchromatin are resilient to these knockdowns. Furthermore, transcriptomic data has revealed hundreds of genes displaying differential expression in these knockdowns and showed that the majority of differentially expressed genes are located within reorganized TADs. Our work identifies a novel and functional role for architectural proteins at TAD borders in Drosophila and a link between TAD reorganization and subsequent changes in gene expression.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research