Open AccessNDF is a transcription factor that stimulates elongation by RNA polymerase II
Author(s) -
Jia Fei,
Jun Xu,
Ziwei Li,
Kevin S. Xu,
Dong Wang,
George A. Kassavetis,
James T. Kadonaga
Publication year - 2022
Publication title -
genes and development
Language(s) - English
Resource type - Journals
eISSN - 1549-5477
pISSN - 0890-9369
DOI - 10.1101/gad.349150.121
Subject(s) - biology , rna polymerase ii , elongation factor , transcription factor ii d , transcription factor , transcription factor ii e , transcription factor ii f , elongation , nucleosome , microbiology and biotechnology , transcription (linguistics) , transcription factor ii b , termination factor , rna polymerase , rna polymerase ii holoenzyme , dna , promoter , gene , rna , gene expression , genetics , transcriptional regulation , chromatin , ribosome , linguistics , materials science , philosophy , ultimate tensile strength , metallurgy
RNA polymerase II (Pol II) elongation is a critical step in gene expression. Here we found that NDF, which was identified as a bilaterian nucleosome-destabilizing factor, is also a Pol II transcription factor that stimulates elongation with plain DNA templates in the absence of nucleosomes. NDF binds directly to Pol II and enhances elongation by a different mechanism than that used by transcription factor TFIIS. Moreover, yeast Pdp3, which is related to NDF, binds to Pol II and stimulates elongation. Thus, NDF is a Pol II binding transcription elongation factor that is localized over gene bodies and is conserved from yeast to humans.