Open AccessCombinatorial impact of osmotic and heat shocks on the composition of membrane lipids and osmolytes in Aspergillus niger
Author(s) -
Elena A. Ianutsevich,
В. М. Терешина
Publication year - 2019
Publication title -
microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.352
H-Index - 35
eISSN - 1465-2080
pISSN - 1350-0872
DOI - 10.1099/mic.0.000796
Subject(s) - trehalose , osmolyte , osmoprotectant , biochemistry , phosphatidic acid , degree of unsaturation , mannitol , membrane , chemistry , composition (language) , membrane lipids , phospholipid , membrane fluidity , biology , chromatography , amino acid , proline , linguistics , philosophy
The combinatorial action of osmotic (OS) and heat (HS) shocks on the composition of soluble cytosol carbohydrates and membrane lipids was studied. For the first time it was demonstrated that the combinatorial effect of these shocks led to the non-additive response - an increase in the trehalose level, characteristic for HS, but at the same time suppression of glycerol production, uncharacteristic of the OS response. In addition, combinatorial action resulted in a new effect - increase in the mannitol level, which was not typical for the individual HS or OS responses. On the contrary, a general pattern of change was observed in the composition of membrane lipids in response to both individual HS and OS, and their combinations, which was a twofold increase in the proportion of phosphatidic acids. At the same time, the mechanism of alteration in the degree of unsaturation of membrane phospholipids was not involved in adaptation. The response to combinatorial shocks includes the accumulation of trehalose and mannitol, and increase in the proportion of phosphatidic acids in membrane lipids.