Open AccessPolymorphisms in the Helicobacter pylori NY43 strain and its prophage-cured derivatives
Author(s) -
Hiroaki Takeuchi,
Mizuki Kira,
Sayuri Konishi,
Jumpei Uchiyama,
Shigenobu Matsuzaki,
Yuichi Matsumura
Publication year - 2018
Publication title -
microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 179
eISSN - 1465-2080
pISSN - 1350-0872
DOI - 10.1099/mic.0.000665
Subject(s) - prophage , helicobacter pylori , biology , strain (injury) , microbiology and biotechnology , bacteriophage , genome , genetics , mutant , gene , escherichia coli , anatomy
This study aimed to determine the characteristics of the Helicobacter pylori host NY43 strain and its prophage-cured derivative. H. pylori colonizing the human stomach cause many diseases. They show high genetic diversity, allowing the development of mutant strains that can form bacterial communities adapted to specific environmental conditions. Bacteriophage activities are associated with bacterial evolution, including pathogenicity development. Herein, we reported the complete genome sequence and genomic organization of two H. pylori prophages, KHP30 and KHP40; the effects of KHP30 on the behaviours of NY43 are not yet known. We showed that approximately 57 % prophage-cured derivatives spontaneously appeared in the exponential phase during liquid culture, and the biological characteristics of these derivatives differed from those of the host NY43. KHP30 reinfected the cured derivatives, and the curing ratio was influenced by culture conditions. KHP30 was shown to promote the development of a flexible H. pylori community with variable characteristics.