Open AccessComparative genomics and evolutionary analysis of Lactococcus garvieae isolated from human endocarditis
Author(s) -
Carlos FrancésCuesta,
Iván Ansari,
J. F. FernándezGarayzábal,
Alicia Gibello,
Fernando Gónzález-Candelas
Publication year - 2022
Publication title -
microbial genomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.476
H-Index - 28
ISSN - 2057-5858
DOI - 10.1099/mgen.0.000771
Subject(s) - biology , lactococcus , endocarditis , microbiology and biotechnology , comparative genomics , genome , genetics , pathogen , plasmid , lactococcus lactis , gene , genomics , bacteria , medicine , lactic acid , surgery
Lactococcus garvieae is a well-known pathogen of fish, but is rarely involved in infections in humans and other mammals. In humans, the main clinical manifestation of L. garvieae infections is endocarditis usually related to the ingestion of contaminated food, such as undercooked fish and shellfish. This study presents the first complete genomic sequence of a clinical L. garvieae strain isolated from a patient with endocarditis and its comparative analysis with other genomes. This human isolate contains a circular chromosome of 2 099 060 bp and one plasmid of 50 557 bp. In comparison with other fully sequenced L. garvieae strains, the chromosomal DNA of L. garvieae Lg-Granada carries a low proportion of insertion sequence elements and a higher number of putative prophages. Our results show that, in general, L. garvieae is a highly recombinogenic species with an open pangenome in which almost 30 % of its genome has undergone horizontal transfers. Within the genus Lactococcus, L. lactis is the main donor of genetic components to L. garvieae but, taking Lg-Granada as a representative, this bacterium tends to import more genes from Bacilli taxa than from other Lactococcus species.