Open AccessMolecular detection of Mycobacterium tuberculosis in poor-quality cough specimens
Author(s) -
Tondani Asaph. Mboneni,
Owen O. Eales,
Ntsoaki Leticia Mosina,
P. Bernard Fourie
Publication year - 2020
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/jmm.0.001239
Subject(s) - genexpert mtb/rif , tuberculosis , mycobacterium tuberculosis , medicine , mycobacterium tuberculosis complex , microbiology and biotechnology , tuberculosis diagnosis , liquid culture , virology , pathology , biology , botany
Clinical specimens unfit for laboratory processing represent missed opportunities for diagnosing tuberculosis. Poor-quality cough specimens ( n =61) from presumptive tuberculosis cases were cultured and GeneXpert MTB/RIF (Xpert) successfully performed on samples transferred by flocked swab into PrimeStore molecular transport medium (PS-MTM). Mycobacterium tuberculosis was grown in culture from 13 (21.3 %) and Xpert reported 15 (24.2 %) positive, of which 10 concordant. RT-PCR of PS-MTM samples showed enhanced sensitivity; three positives were missed by Xpert, five by culture and three more detected for a total of 21 positives (34.4 %).