Molecular detection of Mycobacterium tuberculosis in poor-quality cough specimens | Zendy

Tondani Asaph. Mboneni | Zendy; Owen O. Eales | Zendy; Ntsoaki Leticia Mosina | Zendy; P. Bernard Fourie | Zendy

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Molecular detection of Mycobacterium tuberculosis in poor-quality cough specimens

Author(s) -

Tondani Asaph. Mboneni,

Owen O. Eales,

Ntsoaki Leticia Mosina,

P. Bernard Fourie

Publication year - 2020

Publication title -

journal of medical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.91

H-Index - 117

eISSN - 1473-5644

pISSN - 0022-2615

DOI - 10.1099/jmm.0.001239

Subject(s) - genexpert mtb/rif , tuberculosis , mycobacterium tuberculosis , medicine , mycobacterium tuberculosis complex , microbiology and biotechnology , tuberculosis diagnosis , liquid culture , virology , pathology , biology , botany

Clinical specimens unfit for laboratory processing represent missed opportunities for diagnosing tuberculosis. Poor-quality cough specimens ( n =61) from presumptive tuberculosis cases were cultured and GeneXpert MTB/RIF (Xpert) successfully performed on samples transferred by flocked swab into PrimeStore molecular transport medium (PS-MTM). Mycobacterium tuberculosis was grown in culture from 13 (21.3 %) and Xpert reported 15 (24.2 %) positive, of which 10 concordant. RT-PCR of PS-MTM samples showed enhanced sensitivity; three positives were missed by Xpert, five by culture and three more detected for a total of 21 positives (34.4 %).

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