Open AccessStaphylococcus aureus nasal isolates may have the same genetic profile in atopic dermatitis paediatric patients and their close contacts
Author(s) -
Fernanda Sampaio Cavalcante,
Celina Vieira da Cunha Guedes Alvarenga,
Simone Saintive,
Eliane de Dios Abad,
Dennis de Carvalho Ferreira,
Kátia Regietto dos Santos
Publication year - 2020
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/jmm.0.001197
Subject(s) - staphylococcus aureus , anterior nares , pulsed field gel electrophoresis , atopic dermatitis , microbiology and biotechnology , methicillin resistant staphylococcus aureus , medicine , genotype , population , virulence , pathogen , biology , dermatology , bacteria , gene , genetics , environmental health
Atopic dermatitis (AD) is a chronic skin disease that affects up to 20 % of the paediatric population worldwide. Staphylococcus aureus colonizes anterior nares and can be transmitted in the home environment, aggravating AD. This study aimed to detect S. aureus from nares of AD patients and their family contacts, as well as to evaluate the antimicrobial resistance, virulence and clonality of these isolates. Among the 48 family groups investigated, 30 groups were selected, as both the child and his/her respective contact had methicillin-sensitive S. aureus (MSSA) (24 cases; 54 MSSA isolates) or methicillin-resistant S. aureus (MRSA) isolates (6 cases; 13 MRSA isolates). All MRSA isolates carried SCC mec IV. S. aureus carrying PVL genes were detected in 60 % of patients. Pulsed-field gel electrophoresis (PFGE) analysis was performed for 31 isolates from 15 family groups: all 6 with MRSA and 9 with MSSA isolates. Similar genotypic profiles between isolates from patients and their family contacts were noted in 10 (66.6 %) family groups, 5 (83.3 %) of the MRSA family groups and 5 (55.5 %) of the MSSA family groups, indicating that the pathogen was transmitted through family contacts.