Open AccessIn vitro inhibitory effect of statins on planktonic cells and biofilms of the Sporothrix schenckii species complex
Author(s) -
Raimunda Sâmia Nogueira Brilhante,
Xhaulla Maria Quariguasi Cunha Fonseca,
Vandbergue Santos Pereira,
Géssica dos Santos Araújo,
Jonathas Sales de Oliveira,
Lana Glerieide Silva Garcia,
Anderson Messias Rodrigues,
Zoilo Pires de Camargo,
Waldemiro Aquino Pereira-Neto,
Débora de Souza Collares Maia Castelo-Branco,
Rossana de Aguiar Cordeiro,
José Júlio Costa Sidrim,
Marcos Fábio Gadelha Rocha
Publication year - 2020
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/jmm.0.001195
Subject(s) - sporothrix schenckii , pravastatin , microbiology and biotechnology , atorvastatin , terbinafine , simvastatin , pharmacology , sporothrix , biofilm , broth microdilution , itraconazole , biology , sporotrichosis , chemistry , minimum inhibitory concentration , antimicrobial , cholesterol , antifungal , biochemistry , immunology , bacteria , genetics
. Sporotrichosis, caused by species of the Sporothrix schenckii complex, is the most prevalent subcutaneous mycosis in many areas of Latin America. Statins are a class of drugs widely used for lowering high sterol levels through their action on 3-hydroxy-3-methylglutaryl-CoA reductase, a key enzyme in the synthesis of sterol. Aim. In this study, the antifungal activity of statins (simvastatin, atorvastatin, pravastatin) against planktonic cells and biofilms of S. schenckii complex species was evaluated, as well as the interaction of pravastatin with classical antifungals (amphotericin B, itraconazole, terbinafine). Methodology. Eighteen strains of Sporothrix species were used. The antifungal susceptibility assay was performed using the broth microdilution method. Mature biofilms were exposed to statins and metabolic activity was measured by the XTT reduction assay. Results. MICs of statins ranged from 8 to 512 μg ml −1 and from 8 to 256 μg ml −1 for filamentous and yeast forms, respectively. Regarding mature biofilms, MICs of 50 % inhibition (SMIC50) were 128 μg ml −1 for simvastatin and atorvastatin and >2048 μg ml −1 for pravastatin. MICs of 90 % inhibition (SMIC90) were 512 μg ml −1 for simvastatin and >2048 μg ml −1 for atorvastatin and pravastatin. Conclusion . These results highlight the antifungal and antibiofilm potential of statins against S. schenckii complex species.