Open AccessAntimicrobial activity of the lupane triterpene 3β,6β,16β-trihydroxylup-20(29)-ene isolated from Combretum leprosum Mart.
Author(s) -
Beatriz Gonçalves Cruz,
Alexandre Magno Rodrigues Teixeira,
Priscila Teixeira da Silva,
Mayron Alves de Vasconcelos,
Mayron Alves de Vaconcelos,
Edson Holanda Teixeira,
Hélcio Silva dos Santos,
Paulo Nogueira Bandeira,
Diniz Maciel de Sena-Júnior,
Veruscka Pedrosa Barreto,
Henrique Douglas Melo Coutinho
Publication year - 2019
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/jmm.0.001056
Subject(s) - triterpene , combretaceae , antimicrobial , antibacterial activity , staphylococcus aureus , antibiotics , microbiology and biotechnology , gentamicin , traditional medicine , chemistry , biology , bacteria , medicine , genetics , alternative medicine , pathology
. Combretum leprosum (Combretaceae) is commonly found in the Northeast Region of Brazil and is known for several bioactivities, including antimicrobial ones. Because of increasing bacterial antibiotic resistance, natural products from several plants have been studied as putative adjuvants to antibiotic activity, including products from C. leprosum. Aims . This study was carried out to investigate the structural properties, bactericidal activity and antibiotic modifying action of the lupane triterpene 3β,6β,16β-trihydroxylup-20(29)-ene (CLF1) isolated from C. leprosum Mart. leaves. Methods . The CLF1 was evaluated by the Fourier transform infrared spectroscopy method and the antibacterial activity of this compound was assayed alone and in association with antibiotics by microdilution assay. Results . Spectroscopic studies confirmed the molecular structure of the CLF1 and permitted assignment of the main infrared bands of this natural product. Microbiological assays showed that this lupane triterpene possesses antibacterial action with clinical relevance against Staphylococcus aureus . The CLF1 triterpene increased antimicrobial activity against the multidrug-resistant Escherichia coli 06 strain when associated with the antibiotics gentamicin and amikacin. Synergistic effects were observed against the S. aureus 10 strain in the presence of the CLF1 triterpene with the antibiotic gentamicin. Conclusion . In conclusion, the CLF1 compound may be useful in the development of antibacterial drugs against the aforementioned bacteria.