Open AccessEvaluation of LAMP-based assays for carbapenemase genes
Author(s) -
Surobhi Lahiri,
Rasika Venkataraman,
Akshaya Jagan,
Gargi S. Deshmukh,
Sudipta Patra,
V. Ram Reddy,
V. Sangeetha,
Rachana Solanki,
Jyoti Gupta,
Karuna Patel,
Anuradha De,
Chiranjay Mukhopadhyay,
Mary Dias,
Reba Kanungo,
DK Mendiratta,
Pradeep Nawal,
Jayanthi Shastri,
V Lakshmi,
Radha Rangarajan
Publication year - 2019
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/jmm.0.001050
Subject(s) - gene , biology , computational biology , microbiology and biotechnology , genetics
Purpose . Rapid and accurate detection of carbapenem resistance is a critical requirement for the selection of appropriate therapy and initiation of infection control measures. Although several tests are available, their use is limited by one or more factors. Phenotypic tests are lengthy, have variable sensitivity and specificity and do not generally identify the carbapenemase. Molecular assays overcome many of these issues but cost can be a barrier to adoption, particularly in low-resource settings. To address the need for affordable, molecular tools, we have assessed the performance characteristics of loop-mediated isothermal amplification (LAMP)-based assays for the major carbapenemase genes, bla NDM , bla KPC , bla OXA-48 , bla OXA-23 bla VIM and bla IMP . Methodology . The assays were validated using 1849 Gram-negative Indian clinical isolates obtained from seven hospitals and diagnostic centres. Results . The assays had diagnostic sensitivities of 98.14 %, 98.92 %, 100 %, 98.48 %, and diagnostic specificities of 98.94 %, 99.61 %, 97.42 %, 99.38 % for bla NDM , bla OXA-48 , bla OXA-23 and bla VIM , respectively. Due to a low number of isolates positive for bla KPC and bla IMP , the performance characteristics of assays for these two genes could not be suitably evaluated. Conclusion . The performance characteristics suggest suitability for diagnostic and surveillance purposes.