Open AccessValidation of the Seegene RV15 multiplex PCR for the detection of influenza A subtypes and influenza B lineages during national influenza surveillance in hospitalized adults
Author(s) -
Jason J. LeBlanc,
May ElSherif,
Sunita Mulpuru,
Michelle Warhuus,
Ardith Ambrose,
Melissa K. Andrew,
Guy Boivin,
William Bowie,
Ayman Chit,
Gaël Dos Santos,
K. Green,
Scott A. Halperin,
Todd F. Hatchette,
Barbara Ibarguchi,
Jennie Johnstone,
Kevin Katz,
Joanne M. Langley,
Philippe Lagacé-Wiens,
Mark Loeb,
Arve Lund,
Donna MacKinCameron,
Anne McCarthy,
Janet E. McElhaney,
Allison McGeer,
André Poirier,
Jeff Powis,
David Richardson,
Makeda Semret,
Vivek Shinde,
Daniel Smyth,
Sylvie Trottier,
Louis Valiquette,
Duncan Webster,
L. Ye,
Shelly McNeil
Publication year - 2020
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/jmm.0.001032
Subject(s) - virology , multiplex , multiplex polymerase chain reaction , biology , influenza a virus , real time polymerase chain reaction , virus , polymerase chain reaction , microbiology and biotechnology , gene , genetics
Background . The Serious Outcomes Surveillance Network of the Canadian Immunization Research Network (CIRN SOS) has been performing active influenza surveillance since 2009 (ClinicalTrials.gov identifier: NCT01517191). Influenza A and B viruses are identified and characterized using real-time reverse-transcriptase polymerase chain reaction (RT-PCR), and multiplex testing has been performed on a subset of patients to identify other respiratory virus aetiologies. Since both methods can identify influenza A and B, a direct comparison was performed. Methods . Validated real-time RT-PCRs from the World Health Organization (WHO) to identify influenza A and B viruses, characterize influenza A viruses into the H1N1 or H3N2 subtypes and describe influenza B viruses belonging to the Yamagata or Victoria lineages. In a subset of patients, the Seeplex RV15 One-Step ACE Detection assay (RV15) kit was also used for the detection of other respiratory viruses. Results . In total, 1111 nasopharyngeal swabs were tested by RV15 and real-time RT-PCRs for influenza A and B identification and characterization. For influenza A, RV15 showed 98.0 % sensitivity, 100 % specificity and 99.7 % accuracy. The performance characteristics of RV15 were similar for influenza A subtypes H1N1 and H3N2. For influenza B, RV15 had 99.2 % sensitivity, 100 % specificity and 99.8 % accuracy, with similar assay performance being shown for both the Yamagata and Victoria lineages. Conclusions . Overall, the detection of circulating subtypes of influenza A and lineages of influenza B by RV15 was similar to detection by real-time RT-PCR. Multiplex testing with RV15 allows for a more comprehensive respiratory virus surveillance in hospitalized adults, without significantly compromising the reliability of influenza A or B virus detection.