Open AccessAn identification protocol for ESBL-producing Gram-negative bacteria bloodstream infections using a MinION nanopore sequencer
Author(s) -
Jun Sakai,
Norihito Tarumoto,
Masahiro Kodana,
Sae Ashikawa,
Kazuo Imai,
Tomohiro Kawamura,
Kenji Ikebuchi,
Takashi Murakami,
Kotaro Mitsutake,
Takeshi Maeda,
Shigefumi Maesaki
Publication year - 2019
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/jmm.0.001024
Subject(s) - minion , nanopore sequencing , dna sequencer , biology , microbiology and biotechnology , dna extraction , dna sequencing , bacteria , 16s ribosomal rna , whole genome sequencing , polymerase chain reaction , computational biology , genome , dna , gene , genetics
The new third-generation sequencing platform MinION is an attractive maintenance-free and disposable portable tool that can perform long-read and real-time sequencing. In this study, we validated this technology for the identification of pathogens from positive blood culture (BC) bottles.