Open AccessDetection and identification of bacterial pathogens directly from sputum samples by pyrosequencing
Author(s) -
Jiangfeng Lyu,
Jing Zhang,
Xuyi Ren
Publication year - 2019
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/jmm.0.000917
Subject(s) - pyrosequencing , sputum , microbiology and biotechnology , biology , bacteria , antibiotics , primer (cosmetics) , microbiological culture , lower respiratory tract infection , respiratory tract infections , medicine , tuberculosis , chemistry , gene , respiratory system , genetics , pathology , organic chemistry , anatomy
The standard culture findings for detecting and identifying bacterial pathogens in patients with lower respiratory tract infections (LRTIs) are usually not available for two to three days, which delays the initiation of appropriate antibiotic therapies. We aimed to develop a faster method of identification of bacterial pathogens in LRTIs which would offer a timelier guide to initial antibiotic choices.