Open AccessSynthesis of conjugated PIA–rSesC and immunological evaluation against biofilm-forming Staphylococcus epidermidis
Author(s) -
Bahman Mirzaei,
Seyed Fazlollah Mousavi,
Reyhane Babaei,
Sara Bahonar,
Seyed Davar Siadat,
Mehdi Shafiee Ardestani,
Mohammad Shahrooei,
J. Van Eldere
Publication year - 2019
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/jmm.0.000910
Subject(s) - staphylococcus epidermidis , immunogenicity , microbiology and biotechnology , biofilm , conjugate , antibody , immune system , in vivo , conjugate vaccine , chemistry , immunology , medicine , biology , virology , bacteria , staphylococcus aureus , mathematical analysis , mathematics , genetics
Staphylococcus epidermidis is an opportunistic pathogen and a leading cause of morbidity and premature mortality in patients with medical device-related infections, which is a concern in hospitalized patients. Developing a strategy to raise opsonic antibodies against polysaccharide intracellular adhesin (PIA) could be promising for the elimination of colonizing and biofilm-forming S. epidermidis. Following the purification of truncated rSesC protein and PIA, for the first time, PIA was conjugated to rSesC as a carrier to increase the immunogenicity of PIA and its efficacy in mice was evaluated. The structure of the conjugate was analysed using the Fourier transform infrared spectroscopy (FTIR) and proton nuclear magnetic resonance spectroscopy (H1- NMR) methods. Afterwards, the immune response was evaluated by measuring the total IgG, IgG2a and IgG2b titres.