Open AccessBacteriophage-associated genes responsible for the widely divergent phenotypes of variants of Burkholderia pseudomallei strain MSHR5848
Author(s) -
David DeShazer,
Sean Lovett,
Joshua B. Richardson,
Galina Koroleva,
Kathleen Kuehl,
Kei Amemiya,
Miao Sun,
Patricia L. Worsham,
Susan L. Welkos
Publication year - 2019
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/jmm.0.000908
Subject(s) - biology , burkholderia pseudomallei , gene , phenotype , genetics , melioidosis , bacteriophage , microbiology and biotechnology , bacteria , escherichia coli
Burkholderia pseudomallei, the tier 1 agent of melioidosis, is a saprophytic microbe that causes endemic infections in tropical regions such as South-East Asia and Northern Australia. It is globally distributed, challenging to diagnose and treat, infectious by several routes including inhalation, and has potential for adversarial use. B. pseudomallei strain MSHR5848 produces two colony variants, smooth (S) and rough (R), which exhibit a divergent range of morphological, biochemical and metabolic phenotypes, and differ in macrophage and animal infectivity. We aimed to characterize two major phenotypic differences, analyse gene expression and study the regulatory basis of the variation.