Open AccessA rapid and simple detection method for phenotypic antimicrobial resistance in Escherichia coli by loop-mediated isothermal amplification
Author(s) -
Yusuke Ota,
Kazuki Furuhashi,
Takamasa Nanba,
Katsumasa Yamanaka,
Jinko Ishikawa,
Osanori Nagura,
Etsuko Hamada,
Masato Maekawa
Publication year - 2019
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/jmm.0.000903
Subject(s) - loop mediated isothermal amplification , escherichia coli , microbiology and biotechnology , phenotype , simple (philosophy) , loop (graph theory) , antimicrobial , biology , genetics , gene , mathematics , dna , philosophy , epistemology , combinatorics
In infectious disease therapy, administration of adequate antimicrobial agents is essential for preventing the emergence and spread of resistant bacteria. However, conventional antimicrobial susceptibility testing (AST), based on bacterial growth, is time consuming; therefore, a rapid, simple assay is needed for the timely selection of appropriate antibiotics in clinical laboratories. Here, we established a simple, cost-effective, time-saving and highly sensitive AST assay based on loop-mediated isothermal amplification (LAMP).