Open AccessRapid detection of mcr-1 by recombinase polymerase amplification
Author(s) -
Jialiang Xu,
Xiaoxun Wang,
Yang Long,
Biao Kan,
Xin Lü
Publication year - 2018
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/jmm.0.000865
Subject(s) - recombinase polymerase amplification , plasmid , taqman , biology , microbiology and biotechnology , colistin , polymerase chain reaction , detection limit , gene , recombinase , genetics , chemistry , chromatography , antibiotics , recombination
The plasmid-mediated mcr-1 gene conferring colistin resistance has a strong ability to spread. The objective of this study was to establish a rapid and sensitive recombinase polymerase amplification (RPA) method for plasmid-mediated polymyxin-resistant gene mcr-1 detection.