Open AccessA carbapenem-resistant clinical isolate of Aeromonas hydrophila in Japan harbouring an acquired gene encoding GES-24 β-lactamase
Author(s) -
Kohei Uechi,
Tatsuya Tada,
Yusuke Sawachi,
Tomomi Hishinuma,
Rei Takaesu,
Mika Nakama,
Isamu Nakasone,
Teruo Kirikae,
Jiro Fujita
Publication year - 2018
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/jmm.0.000842
Subject(s) - meropenem , aeromonas hydrophila , imipenem , microbiology and biotechnology , aeromonas , carbapenem , biology , recombinant dna , gene , tetr , bacteria , antibiotics , antibiotic resistance , genetics , repressor , transcription factor
Several species of Aeromonas produce the enzyme CphA metallo-β-lactamase. This study describes an isolate of Aeromonas hydrophila harbouring an acquired gene encoding the carbapenemase GES-24. This isolate was obtained from an inpatient in Okinawa, Japan, with no apparent record of travelling overseas. The minimum inhibitory concentrations of carbapenems against this isolate were 8 µg ml -1 for imipenem and 16 µg ml -1 for meropenem. Recombinant GES-24 hydrolyzed all of the tested β-lactams, including imipenem and meropenem. The genomic environment surrounding blaGES-24 was intI1-blaGES-24-aac(6')-IIc-qacEdelta1-sulI-orfX-tetR-tetE. This is the first report of A. hydrophila producing a GES-type carbapenemase.